Facts About Tissue Fixation Revealed
This really is especially real for immunohistochemistry by which ten% NBF has long been proven to become a weak fixative (Fig. two). Some problems with using 10% NBF to be a fixative are actually corrected via the introduction of other experimental techniques such as antigen retrieval (Shi et al. 2001) and using small amplicons in RT-Q-PCR (Steg et